Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB antibody [HL2879] (GTX640158) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB antibody [HL2879] (GTX640158) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640158/GTX640158_T-45369_20240419_WB_shRNA_watermark_24042400_635.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB antibody [HL2879] (GTX640158) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![CREB antibody [HL2879] detects CREB protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. CREB stained by CREB antibody [HL2879] (GTX640158) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640158/GTX640158_T-45369_20240326_IHC-P_M_24051400_391.webp "CREB antibody [HL2879] detects CREB protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. CREB stained by CREB antibody [HL2879] (GTX640158) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![CREB antibody [HL2879] detects CREB protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat esophagus. CREB stained by CREB antibody [HL2879] (GTX640158) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX640158/GTX640158_T-45369_20240326_IHC-P_R_24051400_421.webp "CREB antibody [HL2879] detects CREB protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat esophagus. CREB stained by CREB antibody [HL2879] (GTX640158) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![CREB antibody [HL2879] detects CREB protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CREB stained by CREB antibody [HL2879] (GTX640158) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640158/GTX640158_T-45369_20240426_ICC_IF_24051400_928.webp "CREB antibody [HL2879] detects CREB protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CREB stained by CREB antibody [HL2879] (GTX640158) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.")
![Various whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB antibody [HL2879] (GTX640158) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640158/GTX640158_45432_20240712_WB_24071520_161.webp "Various whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB antibody [HL2879] (GTX640158) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB antibody [HL2879] (GTX640158) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640158/GTX640158_45432_20240712_WB_M_R_24071520_597.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB antibody [HL2879] (GTX640158) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.")
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB antibody [HL2879] (GTX640158) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
CREB antibody [HL2879]
GTX640158
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCREB1
Overview
- SupplierGeneTex
- Product NameCREB antibody [HL2879]
- Delivery Days Customer7
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2879
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1385
- Target nameCREB1
- Target descriptioncAMP responsive element binding protein 1
- Target synonymsCREB, CREB-1, cyclic AMP-responsive element-binding protein 1, active transcription factor CREB, cAMP-response element-binding protein-1, cyclic adenosine 3',5'-monophosphate response element binding protein, cyclic adenosine 3',5'-monophosphate response element-binding protein CREB, transactivator protein
- HostRabbit
- IsotypeIgG
- Protein IDP16220
- Protein NameCyclic AMP-responsive element-binding protein 1
- Scientific DescriptionThis gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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