Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody [HL2776] (GTX639641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![CREB (phospho Ser133) antibody [HL2776] detects CREB (phospho Ser133) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. CREB (phospho Ser133) stained by CREB (phospho Ser133) antibody [HL2776] (GTX639641) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX639641/GTX639641_T-45299_20240301_IHC-P_24030600_333.webp "CREB (phospho Ser133) antibody [HL2776] detects CREB (phospho Ser133) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. CREB (phospho Ser133) stained by CREB (phospho Ser133) antibody [HL2776] (GTX639641) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody [HL2776] (GTX639641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639641/GTX639641_45369_20240412_WB_treatment_EGF_24041523_241.webp "Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody [HL2776] (GTX639641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody [HL2776] (GTX639641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CREB (phospho Ser133) antibody [HL2776]
GTX639641
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetCREB1
Overview
- SupplierGeneTex
- Product NameCREB (phospho Ser133) antibody [HL2776]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2776
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1385
- Target nameCREB1
- Target descriptioncAMP responsive element binding protein 1
- Target synonymsCREB, CREB-1, cyclic AMP-responsive element-binding protein 1, active transcription factor CREB, cAMP-response element-binding protein-1, cyclic adenosine 3',5'-monophosphate response element binding protein, cyclic adenosine 3',5'-monophosphate response element-binding protein CREB, transactivator protein
- HostRabbit
- IsotypeIgG
- Protein IDP16220
- Protein NameCyclic AMP-responsive element-binding protein 1
- Scientific DescriptionThis gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB antibody [HL2879] (GTX640158) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640158/GTX640158_T-45369_20240412_WB_TPM_watermark_24041523_786.webp)
Product group Antibodies
CREB antibody [HL2879]GTX640158
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ReactivityHuman, Mouse, Rat
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- SizePrice