IHC-P analysis of human bladder and rectal tissue using GTX15731 CREB antibody [LB9]. Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes. Dilution : 1:200
![IHC-P analysis of human tonsil tissue using GTX15731 CREB antibody [LB9]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX15731/GTX15731_1034_IHC-P_w_23060620_158.webp "IHC-P analysis of human tonsil tissue using GTX15731 CREB antibody [LB9]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100")
![WB analysis of 25 ug of various whole cell lysates using GTX15731 CREB antibody [LB9]. Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX15731/GTX15731_1517_WB_w_23060620_430.webp "WB analysis of 25 ug of various whole cell lysates using GTX15731 CREB antibody [LB9]. Dilution : 1:500")
![WB analysis of nuclear extracts (30 microg lysate) of Jurkat (Lane 1), HeLa (Lane 2), K-562 (Lane 3), Hep G2 (Lane 4), NIH/3T3 (Lane 5), C2C12 (Lane 6), U-2 OS (Lane 7), A549 (Lane 8), MDA-MB-231 (Lane 9) and T98G (Lane10) using GTX15731 CREB antibody [LB9]. Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX15731/GTX15731_1518_WB_w_23060620_892.webp "WB analysis of nuclear extracts (30 microg lysate) of Jurkat (Lane 1), HeLa (Lane 2), K-562 (Lane 3), Hep G2 (Lane 4), NIH/3T3 (Lane 5), C2C12 (Lane 6), U-2 OS (Lane 7), A549 (Lane 8), MDA-MB-231 (Lane 9) and T98G (Lane10) using GTX15731 CREB antibody [LB9]. Dilution : 1:500")
![ICC/IF analysis of MDA-MB-231 cells using GTX15731 CREB antibody [LB9]. Panel e shows the no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 5 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX15731/GTX15731_288_ICC-IF_w_23060620_819.webp "ICC/IF analysis of MDA-MB-231 cells using GTX15731 CREB antibody [LB9]. Panel e shows the no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 5 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature")
![ICC/IF analysis of HeLa cells using GTX15731 CREB antibody [LB9]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature Dilution : 1:400 for at least 1 hour at roomtemperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX15731/GTX15731_289_ICC-IF_w_23060620_460.webp "ICC/IF analysis of HeLa cells using GTX15731 CREB antibody [LB9]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature Dilution : 1:400 for at least 1 hour at roomtemperature")
![ICC/IF analysis of HeLa cells using GTX15731 CREB antibody [LB9]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature Dilution : 1:500 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX15731/GTX15731_290_ICC-IF_w_23060620_525.webp "ICC/IF analysis of HeLa cells using GTX15731 CREB antibody [LB9]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature Dilution : 1:500 for at least 1 hour at room temperature")
![ChIP analysis of 2 million HeLa cells using GTX15731 CREB antibody [LB9]. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to promoter of NR4A3, CYR61 gene used as positive control target, and the MYOD, SAT2 used as negative control target.](https://www.genetex.com/upload/website/prouct_img/normal/GTX15731/GTX15731_4_ChIP_w_23060620_127.webp "ChIP analysis of 2 million HeLa cells using GTX15731 CREB antibody [LB9]. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to promoter of NR4A3, CYR61 gene used as positive control target, and the MYOD, SAT2 used as negative control target.")
IHC-P analysis of human bladder and rectal tissue using GTX15731 CREB antibody [LB9]. Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes. Dilution : 1:200
CREB antibody [LB9]
GTX15731
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Primate, Rat
TargetCREB1
Overview
- SupplierGeneTex
- Product NameCREB antibody [LB9]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500. ICC/IF: 5 microg/ml. IHC-P: 1:200. IP: 2 microg. ELISA: 0.1-1 microg/ml. ChIP assay: 2.5 microg/106 cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDLB9
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1385
- Target nameCREB1
- Target descriptioncAMP responsive element binding protein 1
- Target synonymsactive transcription factor CREB; cAMP-response element-binding protein-1; CREB; CREB-1; cyclic adenosine 3',5'-monophosphate response element binding protein; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; cyclic AMP-responsive element-binding protein 1; transactivator protein
- HostMouse
- IsotypeIgG1
- Protein IDP16220
- Protein NameCyclic AMP-responsive element-binding protein 1
- Scientific DescriptionThis gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
- ReactivityHuman, Mouse, Primate, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203