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CREB1 Antibody Pair

CSB-EAP005947
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityBovine, Chicken, Equine, Guinea Pig, Goat, Human
TargetCREB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CREB1 Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.25ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID1385
  • Target name
    CREB1
  • Target description
    cAMP responsive element binding protein 1
  • Target synonyms
    active transcription factor CREB; cAMP-response element-binding protein-1; CREB; CREB-1; cyclic adenosine 3',5'-monophosphate response element binding protein; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; cyclic AMP-responsive element-binding protein 1; transactivator protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16220
  • Protein Name
    Cyclic AMP-responsive element-binding protein 1
  • Reactivity
    Bovine, Chicken, Equine, Guinea Pig, Goat, Human
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of CREB1 using anti-CREB1 antibody (A00577-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CREB1 antigen affinity purified polyclonal antibody (Catalog # A00577-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CREB1 at approximately 43,49 kDa. The expected band size for CREB1 is at 36,37,25 kDa.
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