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Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CREB1 Polyclonal Antibody at dilution 1:30
Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CREB1 Polyclonal Antibody at dilution 1:30
Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CREB1 Polyclonal Antibody at dilution 1:30

CREB1 Polyclonal Antibody

E-AB-10060
Elabscience
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetCREB1
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Overview

  • Supplier
    Elabscience
  • Product Name
    CREB1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1385
  • Target name
    CREB1
  • Target description
    cAMP responsive element binding protein 1
  • Target synonyms
    CREB, CREB-1, cyclic AMP-responsive element-binding protein 1, active transcription factor CREB, cAMP-response element-binding protein-1, cyclic adenosine 3',5'-monophosphate response element binding protein, cyclic adenosine 3',5'-monophosphate response element-binding protein CREB, transactivator protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16220
  • Protein Name
    Cyclic AMP-responsive element-binding protein 1
  • Scientific Description
    This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CREB1 using anti-CREB1 antibody (A00577-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CREB1 antigen affinity purified polyclonal antibody (Catalog # A00577-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CREB1 at approximately 43,49 kDa. The expected band size for CREB1 is at 36,37,25 kDa.
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