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CRH Antibody

ORB253156
Biorbyt
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCRH
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Overview

  • Supplier
    Biorbyt
  • Product Name
    CRH Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID1392
  • Target name
    CRH
  • Target description
    corticotropin releasing hormone
  • Target synonyms
    CRF, CRH1, corticoliberin, corticotropin-releasing factor
  • Host
    Rabbit
  • Protein IDP06850
  • Protein Name
    Corticoliberin
  • Scientific Description
    CRH Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CRF/CRH using anti-CRF/CRH antibody (A00629-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRF/CRH antigen affinity purified polyclonal antibody (A00629-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRF/CRH at approximately 25 kDa. The expected band size for CRF/CRH is at 21 kDa.
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IHC image of CSB-PA005963OA01HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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