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ProductsBack/CRKII Rabbit mAb

CRKII Rabbit mAb

ORB1726924
Biorbyt
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCRK
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Overview

  • Supplier
    Biorbyt
  • Product Name
    CRKII Rabbit mAb
  • Delivery Days Customer
    10
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB:1:500~1:2000ICC/IF:1:50~1:200IP:1:50FC:1:100 FC, ICC, IP, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID1398
  • Target name
    CRK
  • Target description
    CRK proto-oncogene, adaptor protein
  • Target synonyms
    CRKII, p38, adapter molecule crk, proto-oncogene c-Crk, v-crk avian sarcoma virus CT10 oncogene homolog, v-crk sarcoma virus CT10 oncogene-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP46108
  • Protein Name
    Adapter molecule crk
  • Scientific Description
    The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Crk p38 using anti-Crk p38 antibody (PB9101). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Crk p38 antigen affinity purified polyclonal antibody (Catalog # PB9101) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Crk p38 at approximately 38 kDa. The expected band size for Crk p38 is at 34 kDa.
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