Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP5 antibody [N1C1] (GTX112287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![CRMP5 antibody [N1C1] detects CRMP5 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: CRMP5 protein stained by CRMP5 antibody [N1C1] (GTX112287) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX112287/GTX112287_40093_20170824_IFA_w_23060500_962.webp "CRMP5 antibody [N1C1] detects CRMP5 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: CRMP5 protein stained by CRMP5 antibody [N1C1] (GTX112287) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![Mouse tissue extract (5 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP5 antibody [N1C1] (GTX112287) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112287/GTX112287_40093_20171103_WB_M_brain_w_23060500_902.webp "Mouse tissue extract (5 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP5 antibody [N1C1] (GTX112287) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![CRMP5 antibody [N1C1] detects CRMP5 protein at cytoplasm in mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. CRMP5 antibody [N1C1] (GTX112287) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX112287/GTX112287_40093_20150506_IHC_M_w_23060500_170.webp "CRMP5 antibody [N1C1] detects CRMP5 protein at cytoplasm in mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. CRMP5 antibody [N1C1] (GTX112287) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP5 antibody [N1C1] (GTX112287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRMP5 antibody [N1C1]
GTX112287
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDPYSL5
Overview
- SupplierGeneTex
- Product NameCRMP5 antibody [N1C1]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.01 mg/ml
- ConjugateUnconjugated
- Gene ID56896
- Target nameDPYSL5
- Target descriptiondihydropyrimidinase like 5
- Target synonymsCRAM, CRMP-5, CRMP5, CV2, RTSC4, Ulip6, dihydropyrimidinase-related protein 5, CRMP3-associated molecule, DRP-5, ULIP-6, UNC33-like phosphoprotein 6, collapsin response mediator protein-5
- HostRabbit
- IsotypeIgG
- Protein IDQ9BPU6
- Protein NameDihydropyrimidinase-related protein 5
- Scientific DescriptionMembers of the CRMP family, such as DPYSL5, are believed to play a role in growth cone guidance during neural development.[supplied by OMIM]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Zeng HY, Liu Q, Peng XY, et al. Detection of serum anti-retinal antibodies in the Chinese patients with presumed autoimmune retinopathy. Graefes Arch Clin Exp Ophthalmol. 2019,257(8):1759-1764. doi: 10.1007/s00417-019-04359-2Read this paper
- Saito M, Saito W, Kanda A, et al. A case of paraneoplastic optic neuropathy and outer retinitis positive for autoantibodies against collapsin response mediator protein-5, recoverin, and α-enolase. BMC Ophthalmol. 2014,14:5. doi: 10.1186/1471-2415-14-5Read this paper
Datasheet
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