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ProductsBack/CRTC1 / MECT1 / TORC1 Antibody

CRTC1 / MECT1 / TORC1 Antibody

LS-C813144
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCRTC1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CRTC1 / MECT1 / TORC1 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23373
  • Target name
    CRTC1
  • Target description
    CREB regulated transcription coactivator 1
  • Target synonyms
    MAML2, MECT1, Mam-2, TORC-1, TORC1, WAMTP1, CREB-regulated transcription coactivator 1, Mastermind-like protein 2, mucoepidermoid carcinoma translocated protein 1, transducer of regulated cAMP response element-binding protein (CREB) 1
  • Host
    Mouse
  • Isotype
    IgM
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TORC1/CRTC1 using anti-TORC1/CRTC1 antibody (A01951-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TORC1/CRTC1 antigen affinity purified polyclonal antibody (Catalog # A01951-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TORC1/CRTC1 at approximately 78KD. The expected band size for TORC1/CRTC1 is at 78KD.
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