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IP analysis of HeLa cell lysate using GTX64731 CSDE1 antibody. Antibody amount : 3microg / 100microg lysate Dilution : 1:1000
IP analysis of HeLa cell lysate using GTX64731 CSDE1 antibody. Antibody amount : 3microg / 100microg lysate Dilution : 1:1000
IP analysis of HeLa cell lysate using GTX64731 CSDE1 antibody. Antibody amount : 3microg / 100microg lysate Dilution : 1:1000

CSDE1 antibody

GTX64731
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetCSDE1
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Overview

  • Supplier
    GeneTex
  • Product Name
    CSDE1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. IP: 1:20 - 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7812
  • Target name
    CSDE1
  • Target description
    cold shock domain containing E1
  • Target synonyms
    D1S155E, UNR, cold shock domain-containing protein E1, N-ras upstream gene protein, NRAS-related, cold shock domain containing E1, RNA binding, upstream of NRAS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75534
  • Protein Name
    Cold shock domain-containing protein E1
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CSDE1/NRU using anti-CSDE1/NRU antibody (A05114-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSDE1/NRU antigen affinity purified polyclonal antibody (Catalog # A05114-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSDE1/NRU at approximately 89 kDa. The expected band size for CSDE1/NRU is at 89 kDa.
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