More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/G-CSF/CSF3 Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CSF3 Polyclonal Antibody, Unconjugated (bs-1023R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CSF3 Polyclonal Antibody, Unconjugated (bs-1023R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CSF3 Polyclonal Antibody, Unconjugated (bs-1023R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

G-CSF/CSF3 Polyclonal Antibody

BS-1023R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Mouse, Porcine, Rat, Sheep
TargetCSF3
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    G-CSF/CSF3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1440
  • Target name
    CSF3
  • Target description
    colony stimulating factor 3
  • Target synonyms
    C17orf33, CSF3OS, GCSF, granulocyte colony-stimulating factor, filgrastim, granulocyte-colony stimulating factor, lenograstim, pluripoietin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09919
  • Protein Name
    Granulocyte colony-stimulating factor
  • Reactivity
    Bovine, Human, Mouse, Porcine, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer. Yuan SJ et al., 2019 Oct 17, J Nanobiotechnology
    Read this paper
  • Kidney injury enhances renal G-CSF expression and modulates granulopoiesis and human neutrophil CD177 in vivo. Volkmann J et al., 2020 Jan, Clin Exp Immunol
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA06459A0Rb at dilution of 1:20
Product group Antibodies
Cusabio
CSF3 AntibodyCSB-PA06459A0RB
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCSF3
  • SizePrice
Anti-CSF3 Antibody
Product group Antibodies
Antibodies.com
Anti-CSF3 AntibodyA28717
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
MedChem Express
G-CSF Antibody (YA3307, Rabbit)HY-P83562
TargetCSF3
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CSF3 / G-CSF AntibodyLS-C486643
ApplicationsELISA
ReactivityHuman
TargetCSF3
  • SizePrice
Product group Antibodies
Biomatik
Csf3 Polyclonal AntibodyCAC07534
ApplicationsELISA, ImmunoHistoChemistry
TargetCSF3
  • SizePrice
Figure 1. Western blot analysis of G-CSF/CSF3 using anti-G-CSF/CSF3 antibody (PB9563). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-G-CSF/CSF3 antigen affinity purified polyclonal antibody (Catalog # PB9563) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for G-CSF/CSF3 at approximately 22 kDa. The expected band size for G-CSF/CSF3 is at 22 kDa.
Product group Antibodies
Boster Bio
Anti-G-CSF/CSF3 Antibody Picoband(r)PB9563-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCSF3
  • SizePrice
Indirect ELISA analysis was performed by coating the plate with recombinant Human G-CSF protein (active) (GTX138166-pro) (213.9-3.34 nM). Coated protein was probed with G-CSF antibody [GT21] (GTX641067) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.
Product group Antibodies
GeneTex
G-CSF antibody [GT21]GTX641067
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetCSF3
  • SizePrice
Product group Antibodies
Biosynth
GCSF antibody10-7910
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CSF3 Antibody144-66072
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCSF3
  • SizePrice