![Indirect ELISA analysis was performed by coating the plate with recombinant Human G-CSF protein (active) (GTX138166-pro) (213.9-3.34 nM). Coated protein was probed with G-CSF antibody [GT21] (GTX641067) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody. Indirect ELISA analysis was performed by coating the plate with recombinant Human G-CSF protein (active) (GTX138166-pro) (213.9-3.34 nM). Coated protein was probed with G-CSF antibody [GT21] (GTX641067) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641067/GTX641067_45537_20240927_ELISA_Indirect_24100318_780.webp)
Indirect ELISA analysis was performed by coating the plate with recombinant Human G-CSF protein (active) (GTX138166-pro) (213.9-3.34 nM). Coated protein was probed with G-CSF antibody [GT21] (GTX641067) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.
G-CSF antibody [GT21]
GTX641067
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCSF3
Overview
- SupplierGeneTex
- Product NameG-CSF antibody [GT21]
- Delivery Days Customer7
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT21
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1440
- Target nameCSF3
- Target descriptioncolony stimulating factor 3
- Target synonymsC17orf33, CSF3OS, GCSF, granulocyte colony-stimulating factor, filgrastim, granulocyte-colony stimulating factor, lenograstim, pluripoietin
- HostMouse
- IsotypeIgG1
- Protein IDP09919
- Protein NameGranulocyte colony-stimulating factor
- Scientific DescriptionThe protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![Sandwich ELISA detection of recombinant Human G-CSF protein (active) (GTX138166-pro) using antibodies as below. Capture: G-CSF antibody [GT21] (GTX641067) (5 μg/mL) Detection: HRP-conjugated G-CSF antibody [GT22] (GTX641068) (1 μg/mL) Please notice that GTX641068 needs to be conjugated to HRP to function as the detection antibody when paired with GTX641067. Please contact us for custom HRP-conjugated antibody. Sandwich ELISA detection of recombinant Human G-CSF protein (active) (GTX138166-pro) using antibodies as below. Capture: G-CSF antibody [GT21] (GTX641067) (5 μg/mL) Detection: HRP-conjugated G-CSF antibody [GT22] (GTX641068) (1 μg/mL) Please notice that GTX641068 needs to be conjugated to HRP to function as the detection antibody when paired with GTX641067. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641067/GTX641067_45537_20241004_ELISA_PAIR_24100900_368.webp)
![U87-MG whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with G-CSF antibody [GT21] (GTX641067) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. U87-MG whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with G-CSF antibody [GT21] (GTX641067) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641067/GTX641067_45537_20241004_WB_Fraction_24100900_981.webp)




![Sandwich ELISA detection of recombinant Human G-CSF protein (active) (GTX138166-pro) using antibodies as below. Capture: G-CSF antibody [GT21] (GTX641067) (5 μg/mL) Detection: HRP-conjugated G-CSF antibody [GT22] (GTX641068) (1 μg/mL) Please notice that GTX641068 needs to be conjugated to HRP to function as the detection antibody when paired with GTX641067. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641068/GTX641068_45537_20241004_ELISA_PAIR_24100900_391.webp)

![IHC-P analysis of human tonsil tissue using GTX34754 G-CSF antibody [CSF3/900].](https://www.genetex.com/upload/website/prouct_img/normal/GTX34754/GTX34754_20200115_IHC-P_1364_w_23060801_464.webp)