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ProductsBack/CTG-B45d / THAP11 Antibody

CTG-B45d / THAP11 Antibody

LS-C812786
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTHAP11
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CTG-B45d / THAP11 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:10000), Flo (1:200 - 1:400), IF (1:200 - 1:1000), IHC (1:200 - 1:1000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID57215
  • Target name
    THAP11
  • Target description
    THAP domain containing 11
  • Target synonyms
    CTG-B43a; CTG-B45d; HRIHFB2206; RONIN; THAP domain-containing protein 11
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Lane 1: A549 Cell lysates; Lane 2: NIH/3T3 Cell lysates; probed with THAP11 (364CT25.4.2) Monoclonal Antibody, unconjugated (bsm-51114M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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Figure 1. Western blot analysis of THAP11 using anti-THAP11 antibody (A08519-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THAP11 antigen affinity purified polyclonal antibody (Catalog # A08519-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for THAP11 at approximately 50 kDa. The expected band size for THAP11 is at 34 kDa.
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