Bio-Connect

CtIP antibody [19E8]

GTX70264
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, Other Application
Product group Antibodies
TargetRBBP8
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    CtIP antibody [19E8]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:1000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5932
  • Target name
    RBBP8
  • Target description
    RB binding protein 8, endonuclease
  • Target synonyms
    COM1; CTBP-interacting protein; CTIP; DNA endonuclease RBBP8; JWDS; RBBP-8; retinoblastoma binding protein 8; RIM; SAE2; SCKL2; sporulation in the absence of SPO11 protein 2 homolog
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ99708
  • Protein Name
    DNA endonuclease RBBP8
  • Scientific Description
    The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • IKZF1 selectively enhances homologous recombination repair by interacting with CtIP and USP7 in multiple myeloma. Liu M et al., 2022, Int J Biol Sci
    Read more
  • A role for human homologous recombination factors in suppressing microhomology-mediated end joining. Ahrabi S et al., 2016 Jul 8, Nucleic Acids Res
    Read more
  • Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination. Baude A et al., 2016 Mar 18, Nucleic Acids Res
    Read more
  • RB E2F1 complex mediates DNA damage responses through transcriptional regulation of ZBRK1. Liao CC et al., 2010 Oct 22, J Biol Chem
    Read more
  • Expression of PCNA-binding domain of CtIP, a motif required for CtIP localization at DNA replication foci, causes DNA damage and activation of DNA damage checkpoint. Gu B et al., 2009 May 1, Cell Cycle
    Read more
  • CtIP silencing as a novel mechanism of tamoxifen resistance in breast cancer. Wu M et al., 2007 Dec, Mol Cancer Res
    Read more
  • CtIP activates its own and cyclin D1 promoters via the E2F/RB pathway during G1/S progression. Liu F et al., 2006 Apr, Mol Cell Biol
    Read more

Related products

Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetRBBP8
  • SizePrice
Product group Antibodies
Bethyl Laboratories
ApplicationsImmunoPrecipitation
TargetRBBP8
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsWestern Blot
TargetRBBP8
  • SizePrice
Product group Antibodies
Antibodies.com
ApplicationsELISA, ImmunoHistoChemistry
  • SizePrice
IHC-P analysis of rat spleen tissue using GTX31413 CtIP antibody. Working concentration : 5 microg/ml
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetRBBP8
  • SizePrice
Product group Antibodies
Cusabio
RBBP8 AntibodyCSB-PA040180
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetRBBP8
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsELISA, ImmunoHistoChemistry
TargetRBBP8
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
TargetRBBP8
  • SizePrice
Immunohistochemical staining of human rectum shows moderate cytoplasmic and nuclear positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoHistoChemistry
TargetRBBP8
  • SizePrice
Figure 1. Western blot analysis of RBBP8 using anti-RBBP8 antibody (A02076-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBBP8 antigen affinity purified polyclonal antibody (Catalog # A02076-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBBP8 at approximately 130 kDa. The expected band size for RBBP8 is at 102 kDa.
Product group Antibodies
Boster Bio
Anti-RBBP8 Antibody Picoband(r)A02076-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetRBBP8
  • SizePrice