FACS analysis of human PBMC stimulated overnight with PMA and Ionomycin stained APC anti-human CD4 antibody, followed by intracellular staining with GTX00492-08 CTLA4 antibody [BNI3] (PE). Right panel : Co-stained with APC anti-human CD4 antibody and GTX00492-08 Left panel : Co-stained with APC anti-human CD4 antibody and PE mouse IgG2a isotype control Antibody amount : 0.5 microg
FACS analysis of human PBMC stimulated overnight with PMA and Ionomycin stained APC anti-human CD4 antibody, followed by intracellular staining with GTX00492-08 CTLA4 antibody [BNI3] (PE). Right panel : Co-stained with APC anti-human CD4 antibody and GTX00492-08 Left panel : Co-stained with APC anti-human CD4 antibody and PE mouse IgG2a isotype control Antibody amount : 0.5 microg
CTLA4 antibody [BNI3] (PE)
GTX00492-08
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman, Monkey, Primate
TargetCTLA4
Overview
- SupplierGeneTex
- Product NameCTLA4 antibody [BNI3] (PE)
- Delivery Days Customer9
- Application Supplier NoteFACS: 0.5 microg (5 microl) for 105-108 cells in 100 microl sample per test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDBNI3
- Concentration0.1 mg/ml
- ConjugateRPE
- FormulationLiquid
- Gene ID1493
- Target nameCTLA4
- Target descriptioncytotoxic T-lymphocyte associated protein 4
- Target synonymsALPS5; CD; CD152; celiac disease 3; CELIAC3; CTLA-4; cytotoxic T lymphocyte associated antigen 4 short spliced form; cytotoxic T-lymphocyte protein 4; cytotoxic T-lymphocyte-associated serine esterase-4; GRD4; GSE; IDDM12; insulin-dependent diabetes mellitus 12; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4
- HostMouse
- IsotypeIgG2a
- Protein IDP16410
- Protein NameCytotoxic T-lymphocyte protein 4
- Scientific DescriptionThis gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Monkey, Primate
- Storage Instruction2°C to 8°C
- UNSPSC12352203
References
- Enhance the anti-renca carcinoma effect of a DNA vaccine targeting G250 gene by co-expression with cytotoxic T-lymphocyte associated antigen-4(CTLA-4). Zhao Y et al., 2017 Jun, Biomed PharmacotherRead more
- Follicular Regulatory CD8 T Cells Impair the Germinal Center Response in SIV and Ex Vivo HIV Infection. Miles B et al., 2016 Oct, PLoS PathogRead more
- Impaired proteasome function activates GATA3 in T cells and upregulates CTLA-4: relevance for Sezary syndrome. Gibson HM et al., 2013 Jan, J Invest DermatolRead more
- Regulatory T cells control HIV replication in activated T cells through a cAMP-dependent mechanism. Moreno-Fernandez ME et al., 2011 May 19, BloodRead more
- CD4(+) regulatory T cells in a cynomolgus macaque model of Mycobacterium tuberculosis infection. Green AM et al., 2010 Aug 15, J Infect DisRead more
- An engineered lipocalin specific for CTLA-4 reveals a combining site with structural and conformational features similar to antibodies. Schoenfeld D et al., 2009 May 19, Proc Natl Acad Sci U S ARead more
- Evaluation of FoxP3 expression in peripheral T-cell lymphoma. Bonzheim I et al., 2008 Oct, Am J Clin PatholRead more
- The inhibitory receptor LILRB1 modulates the differentiation and regulatory potential of human dendritic cells. Young NT et al., 2008 Mar 15, BloodRead more
- Identification and functional characterization of human CD4(+)CD25(+) T cells with regulatory properties isolated from peripheral blood. Jonuleit H et al., 2001 Jun 4, J Exp MedRead more
- Cutting edge: a soluble form of CTLA-4 in patients with autoimmune thyroid disease. Oaks MK et al., 2000 May 15, J ImmunolRead more