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Mouse lung lysate probed with Anti CTLA4 Polyclonal Antibody, Unconjugated (bs-10006R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 34kD. Observed band size: 34kD
Mouse lung lysate probed with Anti CTLA4 Polyclonal Antibody, Unconjugated (bs-10006R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 34kD. Observed band size: 34kD
Mouse lung lysate probed with Anti CTLA4 Polyclonal Antibody, Unconjugated (bs-10006R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 34kD. Observed band size: 34kD

CTLA4 Polyclonal Antibody

BS-10006R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCTLA4
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Overview

  • Supplier
    Bioss
  • Product Name
    CTLA4 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IF(IHC-P)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1493
  • Target name
    CTLA4
  • Target description
    cytotoxic T-lymphocyte associated protein 4
  • Target synonyms
    ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12, cytotoxic T-lymphocyte protein 4, celiac disease 3, cytotoxic T lymphocyte associated antigen 4 short spliced form, cytotoxic T-lymphocyte-associated serine esterase-4, gluten-sensitive enteropathy, insulin-dependent diabetes mellitus 12, ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16410
  • Protein Name
    Cytotoxic T-lymphocyte protein 4
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Immune checkpoints programmed death 1 ligand 1 and cytotoxic T lymphocyte associated molecule 4 in gastric adenocarcinoma. Schloebetaer HA et al., 2016 May, Oncoimmunology
    Read more

Datasheet

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Figure 1. IHC analysis of CTLA4 using anti-CTLA4 antibody (A00020-2). CTLA4 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-CTLA4 Antibody (A00020-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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