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Western Blot analysis of A431 cell using CTNNA1 Polyclonal Antibody at dilution of 1:600
Western Blot analysis of A431 cell using CTNNA1 Polyclonal Antibody at dilution of 1:600
Western Blot analysis of A431 cell using CTNNA1 Polyclonal Antibody at dilution of 1:600

CTNNA1 Polyclonal Antibody

E-AB-10207
Elabscience
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCTNNA1
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Overview

  • Supplier
    Elabscience
  • Product Name
    CTNNA1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1495
  • Target name
    CTNNA1
  • Target description
    catenin alpha 1
  • Target synonyms
    CAP102, MDBS2, MDPT2, catenin alpha-1, alpha-E-catenin, catenin (cadherin-associated protein), alpha 1, 102kDa, epididymis secretory sperm binding protein, renal carcinoma antigen NY-REN-13
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35221
  • Protein Name
    Catenin alpha-1
  • Scientific Description
    Catenin alpha-1is aproteinthat in humans is encoded by theCTNNA1gene. Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be mucHuman, Mouseore dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of CTNNA1 using anti-CTNNA1 antibody (PB9137). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human CTNNA1 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNA1 antigen affinity purified polyclonal antibody (Catalog # PB9137) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNA1 at approximately 47KD. The expected band size for CTNNA1 is at 47KD.
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