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CTNND1 / p120 Catenin Antibody

LS-C331607
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetCTNND1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CTNND1 / p120 Catenin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 65-72kDa/90-108kDa, while the observed MW by Western blot was 105kDa.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    ICC (1:50 - 1:200), IF (1:50 - 1:200), IHC (1:50 - 1:200), IP (1:50 - 1:100), WB (1:500 - 1:2000) The predicted MW is 65-72kDa/90-108kDa, while the observed MW by Western blot was 105kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1500
  • Target name
    CTNND1
  • Target description
    catenin delta 1
  • Target synonyms
    BCDS2; cadherin-associated Src substrate; CAS; catenin (cadherin-associated protein), delta 1; catenin delta-1; CTNND; p120; p120 catenin; p120(CAS); p120(CTN); P120CAS; P120CTN
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of delta 1 Catenin/CAS/CTNND1 using anti-delta 1 Catenin/CAS/CTNND1 antibody (A02333-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: rat RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-delta 1 Catenin/CAS/CTNND1 antigen affinity purified polyclonal antibody (Catalog # A02333-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for delta 1 Catenin/CAS/CTNND1 at approximately 100-110 kDa. The expected band size for delta 1 Catenin/CAS/CTNND1 is at 108 kDa.
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