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Western Blot analysis of H460 K562 cells using Cathepsin L Polyclonal Antibody
Western Blot analysis of H460 K562 cells using Cathepsin L Polyclonal Antibody
Western Blot analysis of H460 K562 cells using Cathepsin L Polyclonal Antibody

CTSL Antibody

CSB-PA004835
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCTSL
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Overview

  • Supplier
    Cusabio
  • Product Name
    CTSL Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1514
  • Target name
    CTSL
  • Target description
    cathepsin L
  • Target synonyms
    cathepsin L1; CATL; CTSL1; major excreted protein; MEP; procathepsin L
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07711
  • Protein Name
    Cathepsin L1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cathepsin L/MEP/CTSL using anti-Cathepsin L/MEP/CTSL antibody (A01589-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HCCP tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin L/MEP/CTSL antigen affinity purified polyclonal antibody (Catalog # A01589-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin L/MEP/CTSL at approximately 37 kDa. The expected band size for Cathepsin L/MEP/CTSL is at 37 kDa.
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