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ProductsBack/Cullin 4A Recombinant Antibody, AbBy Fluor-405 Conjugated

Cullin 4A Recombinant Antibody, AbBy Fluor-405 Conjugated

BSM-61837R-BF405
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetCUL4A
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Overview

  • Supplier
    Bioss
  • Product Name
    Cullin 4A Recombinant Antibody, AbBy Fluor-405 Conjugated
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IF(ICC)(1:50-200), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID8451
  • Target name
    CUL4A
  • Target description
    cullin 4A
  • Target synonyms
    cullin-4A, CUL-4A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13619
  • Protein Name
    Cullin-4A
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cullin 4a using anti-Cullin 4a antibody (A01579-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human MDA-MB-231 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cullin 4a antigen affinity purified polyclonal antibody (Catalog # A01579-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cullin 4a at approximately 88KD. The expected band size for Cullin 4a is at 88KD.
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