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ProductsBack/Cullin 4a antibody [N1C1]
Cullin 4aantibody detects Cullin 4a protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. Cullin 4aantibody (GTX113876) dilution: 1:200.
Cullin 4aantibody detects Cullin 4a protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. Cullin 4aantibody (GTX113876) dilution: 1:200.
Cullin 4aantibody detects Cullin 4a protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. Cullin 4aantibody (GTX113876) dilution: 1:200.

Cullin 4a antibody [N1C1]

GTX113876
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Zebra Fish
TargetCUL4A
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Overview

  • Supplier
    GeneTex
  • Product Name
    Cullin 4a antibody [N1C1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8451
  • Target name
    CUL4A
  • Target description
    cullin 4A
  • Target synonyms
    cullin-4A, CUL-4A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13619
  • Protein Name
    Cullin-4A
  • Scientific Description
    CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins (Liu et al., 2009 [PubMed 19481525]).[supplied by OMIM]
  • Reactivity
    Human, Zebra Fish
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Lanzafame M, Branca G, Landi C, et al. Cockayne syndrome group A and ferrochelatase finely tune ribosomal gene transcription and its response to UV irradiation. Nucleic Acids Res. 2021,49(19):10911-10930. doi: 10.1093/nar/gkab819
    Read this paper
  • Fu SJ, Hu MC, Peng YJ, et al. CUL4-DDB1-CRBN E3 Ubiquitin Ligase Regulates Proteostasis of ClC-2 Chloride Channels: Implication for Aldosteronism and Leukodystrophy. Cells. 2020,9(6). doi: 10.3390/cells9061332
    Read this paper
  • Spatola BN, Lo JY, Wang B, et al. Nuclear and cytoplasmic WDR-23 isoforms mediate differential effects on GEN-1 and SKN-1 substrates. Sci Rep. 2019,9(1):11783. doi: 10.1038/s41598-019-48286-y
    Read this paper
  • Lo JY, Spatola BN, Curran SP. WDR23 regulates NRF2 independently of KEAP1. PLoS Genet. 2017,13(4):e1006762. doi: 10.1371/journal.pgen.1006762
    Read this paper
  • Chen YA, Peng YJ, Hu MC, et al. The Cullin 4A/B-DDB1-Cereblon E3 Ubiquitin Ligase Complex Mediates the Degradation of CLC-1 Chloride Channels. Sci Rep. 2015,5:10667. doi: 10.1038/srep10667
    Read this paper

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Figure 1. Western blot analysis of Cullin 4a using anti-Cullin 4a antibody (A01579-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human MDA-MB-231 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cullin 4a antigen affinity purified polyclonal antibody (Catalog # A01579-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cullin 4a at approximately 88KD. The expected band size for Cullin 4a is at 88KD.
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