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ProductsBack/Anti-Alkaline Phosphatase ALPL Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human liver, using Alkaline Phosphatase Antibody.
Immunohistochemical analysis of paraffin-embedded human liver, using Alkaline Phosphatase Antibody.
Immunohistochemical analysis of paraffin-embedded human liver, using Alkaline Phosphatase Antibody.

Anti-Alkaline Phosphatase ALPL Rabbit Monoclonal Antibody

M01008-1
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetALPL
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Alkaline Phosphatase Rabbit Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    DBF-1
  • Gene ID249
  • Target name
    ALPL
  • Target description
    alkaline phosphatase, biomineralization associated
  • Target synonyms
    alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; APTNAP; AP-TNAP; HOPS; HPPA; HPPC; HPPI; HPPO; liver/bone/kidney-type alkaline phosphatase; tissue non-specific alkaline phosphatase; tissue-nonspecific ALP; TNALP; TNAP; TNSALP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05186
  • Protein Name
    Alkaline phosphatase, tissue-nonspecific isozyme
  • Scientific Description
    Boster Bio Anti-Alkaline Phosphatase ALPL Rabbit Monoclonal Antibody catalog # M01008-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Network pharmacological study and in vitro studies validation-Molecular dynamics simulation of Cistanche deserticola in promoting periodontitis and bone remodeling.
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  • Preparation of an injectable and photocurable carboxymethyl cellulose/hydroxyapatite composite and its application in cranial regeneration.
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  • Nanosized Silk-Magnesium Complexes for Promotion of Angiogenic and Osteogenic Activities.
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  • Explorating the mechanism of Epimedii folium-Rhizoma drynariae herbal pair promoted bone defects healing through network pharmacology and experimental studies.
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  • Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels.
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  • Bone morphogenetic protein 7 promotes odontogenic differentiation of dental pulp stem cells in vitro. Zhu L et al., 2018 Jun 1, Life Sci
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Figure 1. Western blot analysis of Alkaline phosphatase/ALPL using anti-Alkaline phosphatase/ALPL antibody (A01008-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alkaline phosphatase/ALPL antigen affinity purified polyclonal antibody (Catalog # A01008-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alkaline phosphatase/ALPL at approximately 80KD. The expected band size for Alkaline phosphatase/ALPL is at 57KD.
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