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ProductsBack/Anti-Caspase-8 CASP8 Rabbit Monoclonal Antibody
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Anti-Caspase-8 CASP8 Rabbit Monoclonal Antibody

M00042
Boster Bio
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetCASP8
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Caspase-8 CASP8 Rabbit Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    EEG-3
  • Gene ID841
  • Target name
    CASP8
  • Target description
    caspase 8
  • Target synonyms
    ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5, caspase-8, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MACH-alpha-1/2/3 protein, MACH-beta-1/2/3/4 protein, MORT1-associated ced-3 homolog, apoptotic cysteine protease, apoptotic protease Mch-5, caspase 8, apoptosis-related cysteine peptidase, caspase 8, apoptosis-related cysteine protease
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14790
  • Protein Name
    Caspase-8
  • Scientific Description
    Boster Bio Anti-Caspase-8 CASP8 Rabbit Monoclonal Antibody catalog # M00042. Tested in WB application. This antibody reacts with Human.
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Fu X, Mao Q, Zhang B, et al. Thiazolidinedione-Based Structure Modification of Celastrol Provides Thiazolidinedione-Conjugated Derivatives as Potent Agents against Non-Small-Cell Lung Cancer Cells through a Mitochondria-Mediated Apoptotic Pathway. J Nat Prod. 2022,85(4):1147-1156. doi: 10.1021/acs.jnatprod.2c00104
    Read this paper
  • Xing JJ, Hou JG, Liu Y, et al. Supplementation of Saponins from Leaves of Panax quinquefolius Mitigates Cisplatin-Evoked Cardiotoxicity via Inhibiting Oxidative Stress-Associated Inflammation and Apoptosis in Mice. Antioxidants (Basel). 2019,8(9). doi: 10.3390/antiox8090347
    Read this paper
  • Shen J, Cao S, Sun X, et al. Sinoporphyrin Sodium-Mediated Sonodynamic Therapy Inhibits RIP3 Expression and Induces Apoptosis in the H446 Small Cell Lung Cancer Cell Line. Cell Physiol Biochem. 2018,51(6):2938-2954. doi: 10.1159/000496045
    Read this paper
  • Mi XJ, Hou JG, Wang Z, et al. The protective effects of maltol on cisplatin-induced nephrotoxicity through the AMPK-mediated PI3K/Akt and p53 signaling pathways. Sci Rep. 2018,8(1):15922. doi: 10.1038/s41598-018-34156-6
    Read this paper
  • Wu X, Xu Y, Zhu B, et al. Resveratrol induces apoptosis in SGC-7901 gastric cancer cells. Oncol Lett. 2018,16(3):2949-2956. doi: 10.3892/ol.2018.9045
    Read this paper
  • Guan YQ, Li Z, Chen J, et al. Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs. Biomaterials. 2011,32(14):3637-46. doi: 10.1016/j.biomaterials.2011.01.060
    Read this paper

Datasheet

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MSDS

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Figure 1. Western blot analysis of Caspase 8 using anti-Caspase 8 antibody (A00042-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 8 antigen affinity purified polyclonal antibody (Catalog # A00042-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase 8 at approximately 55KD. The expected band size for Caspase 8 is at 55KD.
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