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ProductsBack/GALT antibody [N3C3]

GALT antibody [N3C3]

GTX113702
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetGALT
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Overview

  • Supplier
    GeneTex
  • Product Name
    GALT antibody [N3C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2592
  • Target name
    GALT
  • Target description
    galactose-1-phosphate uridylyltransferase
  • Target synonyms
    gal-1-P uridylyltransferase; galactose-1-phosphate uridyl transferase; galactose-1-phosphate uridylyltransferase; UDP-glucose--hexose-1-phosphate uridylyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07902
  • Protein Name
    Galactose-1-phosphate uridylyltransferase
  • Scientific Description
    Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GALT using anti-GALT antibody (A01460-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 cell lysates, Lane 2: human MCF-7 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALT antigen affinity purified polyclonal antibody (Catalog # A01460-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GALT at approximately 48KD. The expected band size for GALT is at 43KD.
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