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Western Blot analysis of MOUSE-BRAIN cells using Fusin Polyclonal Antibody
Western Blot analysis of MOUSE-BRAIN cells using Fusin Polyclonal Antibody
Western Blot analysis of MOUSE-BRAIN cells using Fusin Polyclonal Antibody

CXCR4 Antibody

CSB-PA002588
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCXCR4
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Overview

  • Supplier
    Cusabio
  • Product Name
    CXCR4 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7852
  • Target name
    CXCR4
  • Target description
    C-X-C motif chemokine receptor 4
  • Target synonyms
    CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS, WHIMS1, C-X-C chemokine receptor type 4, CD184 antigen, LPS-associated protein 3, SDF-1 receptor, chemokine (C-X-C motif) receptor 4, fusin, leukocyte-derived seven transmembrane domain receptor, lipopolysaccharide-associated protein 3, neuropeptide Y receptor Y3, neuropeptide Y3 receptor, seven transmembrane helix receptor, seven-transmembrane-segment receptor, spleen, stromal cell-derived factor 1 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP61073
  • Protein Name
    C-X-C chemokine receptor type 4
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CXCR4 using anti-CXCR4 antibody (A00031-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR4 antigen affinity purified polyclonal antibody (Catalog # A00031-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR4 at approximately 36-40KD. The expected band size for CXCR4 is at 40KD.
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