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CXCR5 Antibody (clone 2C1)

LS-C196755
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCXCR5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CXCR5 Antibody (clone 2C1)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Sandwich ELISA: Recombinant protein. Western Blot: Recombinant protein.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB Sandwich ELISA: Recombinant protein. Western Blot: Recombinant protein.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2C1
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID643
  • Target name
    CXCR5
  • Target description
    C-X-C motif chemokine receptor 5
  • Target synonyms
    BLR1; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5); Burkitt lymphoma receptor 1, GTP-binding protein; CD185; chemokine (C-X-C motif) receptor 5; C-X-C chemokine receptor type 5; CXC-R5; CXCR-5; MDR15; MDR-15; monocyte-derived receptor 15
  • Host
    Mouse
  • Isotype
    IgG2a
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CXCR5 using anti-CXCR5 antibody (A00663-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR5 antigen affinity purified polyclonal antibody (Catalog # A00663-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR5 at approximately 50 kDa. The expected band size for CXCR5 is at 42 kDa.
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