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Lane 1: Mouse lymph node lysates probed with CXCR5 Polyclonal Antibody, Unconjugated (bs-3598R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse lymph node lysates probed with CXCR5 Polyclonal Antibody, Unconjugated (bs-3598R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse lymph node lysates probed with CXCR5 Polyclonal Antibody, Unconjugated (bs-3598R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.

CXCR5 Polyclonal Antibody

BS-3598R
Bioss
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Mouse, Porcine, Rabbit, Rat
TargetCXCR5
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Overview

  • Supplier
    Bioss
  • Product Name
    CXCR5 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID643
  • Target name
    CXCR5
  • Target description
    C-X-C motif chemokine receptor 5
  • Target synonyms
    BLR1, CD185, MDR15, C-X-C chemokine receptor type 5, Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5), Burkitt lymphoma receptor 1, GTP-binding protein, CXC-R5, CXCR-5, MDR-15, chemokine (C-X-C motif) receptor 5, monocyte-derived receptor 15
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP32302
  • Protein Name
    C-X-C chemokine receptor type 5
  • Reactivity
    Bovine, Human, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Gong Q, Zhu Y, Pang N, et al. Increased levels of CCR7(lo)PD-1(hi) CXCR5(+) CD4(+) T cells, and associated factors Bcl-6, CXCR5, IL-21 and IL-6 contribute to repeated implantation failure. Exp Ther Med. 2017,14(6):5931-5941. doi: 10.3892/etm.2017.5334
    Read this paper
  • Huang H, Liu Y, Wang L, et al. Age-related macular degeneration phenotypes are associated with increased tumor necrosis-alpha and subretinal immune cells in aged Cxcr5 knockout mice. PLoS One. 2017,12(3):e0173716. doi: 10.1371/journal.pone.0173716
    Read this paper

Datasheet

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Figure 1. Western blot analysis of CXCR5 using anti-CXCR5 antibody (A00663-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR5 antigen affinity purified polyclonal antibody (Catalog # A00663-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR5 at approximately 50 kDa. The expected band size for CXCR5 is at 42 kDa.
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