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ProductsBack/Cyclin D3 (CCND3) Polyclonal Antibody

Cyclin D3 (CCND3) Polyclonal Antibody

CAU27591
Biomatik
ApplicationsWestern Blot
Product group Antibodies
TargetCCND3
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Overview

  • Supplier
    Biomatik
  • Product Name
    Cyclin D3 (CCND3) Polyclonal Antibo
  • Delivery Days Customer
    12
  • Applications
    Western Blot
  • Applications Supplier
    WB; ICC; IHC-P; IHC-F; ELISA.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID896
  • Target name
    CCND3
  • Target description
    cyclin D3
  • Target synonyms
    D3-type cyclin; G1/S-specific cyclin-D3
  • Host
    Rabbit
  • Protein IDP30281
  • Protein Name
    G1/S-specific cyclin-D3
  • Scientific Description
    The Cyclin D3 (CCND3) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cyclin D3 using anti-Cyclin D3 antibody (PB9103). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human Cyclin D3 protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin D3 antigen affinity purified polyclonal antibody (Catalog # PB9103) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin D3 at approximately 25KD. The expected band size for Cyclin D3 is at 25KD.
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