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Western Blot analysis of K562 cells using CYP11A1 Polyclonal Antibody
Western Blot analysis of K562 cells using CYP11A1 Polyclonal Antibody
Western Blot analysis of K562 cells using CYP11A1 Polyclonal Antibody

CYP11A1 Antibody

CSB-PA005154
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCYP11A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CYP11A1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1583
  • Target name
    CYP11A1
  • Target description
    cytochrome P450 family 11 subfamily A member 1
  • Target synonyms
    cholesterol 20-22 desmolase; cholesterol monooxygenase (side-chain cleaving); cholesterol side-chain cleavage enzyme, mitochondrial; CYP11A; CYPXIA1; cytochrome P450 11A1; cytochrome P450 family 11 subfamily A polypeptide 1; cytochrome P450(scc); cytochrome P450, subfamily XIA (cholesterol side chain cleavage); P450SCC; steroid 20-22-lyase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05108
  • Protein Name
    Cholesterol side-chain cleavage enzyme, mitochondrial
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CYP11A1 using anti-CYP11A1 antibody (A01071-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat ovary tissue lysates, Lane 3: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP11A1 antigen affinity purified polyclonal antibody (Catalog # A01071-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP11A1 at approximately 50 kDa. The expected band size for CYP11A1 is at 50 kDa.
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