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Mouse placenta lysates probed with Rabbit Anti-CYP11A1 Polyclonal Antibody, Unconjugated (bs-3608R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Mouse placenta lysates probed with Rabbit Anti-CYP11A1 Polyclonal Antibody, Unconjugated (bs-3608R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Mouse placenta lysates probed with Rabbit Anti-CYP11A1 Polyclonal Antibody, Unconjugated (bs-3608R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.

CYP11A1 Polyclonal Antibody

BS-3608R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCYP11A1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    CYP11A1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1583
  • Target name
    CYP11A1
  • Target description
    cytochrome P450 family 11 subfamily A member 1
  • Target synonyms
    cholesterol 20-22 desmolase; cholesterol monooxygenase (side-chain cleaving); cholesterol side-chain cleavage enzyme, mitochondrial; CYP11A; CYPXIA1; cytochrome P450 11A1; cytochrome P450 family 11 subfamily A polypeptide 1; cytochrome P450(scc); cytochrome P450, subfamily XIA (cholesterol side chain cleavage); P450SCC; steroid 20-22-lyase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05108
  • Protein Name
    Cholesterol side-chain cleavage enzyme, mitochondrial
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Icariin protects mouse Leydig cell testosterone synthesis from the adverse effects of di(2-ethylhexyl) phthalate. Sun J et al., 2019 Sep 1, Toxicol Appl Pharmacol
    Read more
  • Vitamin D3 mediated regulation of steroidogenesis mitigates testicular activity in an aged rat model. Jeremy M et al., 2019 Jun, J Steroid Biochem Mol Biol
    Read more
  • Melatonin Stimulates STAR Expression and Progesterone Production via Activation of the PI3K/AKT Pathway in Bovine Theca Cells. Wang X et al., 2019, Int J Biol Sci
    Read more
  • Application of rat cryptorchidism model for the evaluation of mesenchymal stromal cell secretome regenerative potential. Sagaradze GD et al., 2019 Jan, Biomed Pharmacother
    Read more
  • Dual effects of sulfasalazine on rat sperm characteristics, spermatogenesis, and steroidogenesis in two experimental models. Ommati MM et al., 2018 Mar 1, Toxicol Lett
    Read more

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Figure 1. Western blot analysis of CYP11A1 using anti-CYP11A1 antibody (A01071-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat ovary tissue lysates, Lane 3: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP11A1 antigen affinity purified polyclonal antibody (Catalog # A01071-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP11A1 at approximately 50 kDa. The expected band size for CYP11A1 is at 50 kDa.
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