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Western blot All lanes: CYP17A1 antibody at 2microg/ml Lane 1: Hela whole cell lysate Lane 2: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa
Western blot All lanes: CYP17A1 antibody at 2microg/ml Lane 1: Hela whole cell lysate Lane 2: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa
Western blot All lanes: CYP17A1 antibody at 2microg/ml Lane 1: Hela whole cell lysate Lane 2: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa

CYP17A1 Antibody

CSB-PA006392EA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCYP17A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CYP17A1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1586
  • Target name
    CYP17A1
  • Target description
    cytochrome P450 family 17 subfamily A member 1
  • Target synonyms
    CPT7, CYP17, P450C17, S17AH, steroid 17-alpha-hydroxylase/17,20 lyase, 17-alpha-hydroxyprogesterone aldolase, CYPXVII, cytochrome P450 17A1, cytochrome P450, family 17, subfamily A, polypeptide 1, cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasia, cytochrome P450-C17, cytochrome P450c17, cytochrome p450 XVIIA1, steroid 17-alpha-monooxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05093
  • Protein Name
    Steroid 17-alpha-hydroxylase/17,20 lyase
  • Scientific Description
    Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 2. IHC analysis of CYP17A1 using anti-CYP17A1 antibody (A00615-1). CYP17A1 was detected in paraffin-embedded section of human testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-CYP17A1 Antibody (A00615-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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