Paraformaldehyde-fixed, paraffin embedded (rat testis）; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome P450 17A1) Polyclonal Antibody, Unconjugated (bs-6695R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis）; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome P450 17A1) Polyclonal Antibody, Unconjugated (bs-6695R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Cytochrome P450 17A1 Polyclonal Antibody
BS-6695R
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Equine, Human, Mouse, Rabbit, Rat, Sheep, Other Species
TargetCYP17A1
Overview
- SupplierBioss
- Product NameCytochrome P450 17A1 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(WB=1:500-2000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:100-500)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID1586
- Target nameCYP17A1
- Target descriptioncytochrome P450 family 17 subfamily A member 1
- Target synonymsCPT7, CYP17, P450C17, S17AH, steroid 17-alpha-hydroxylase/17,20 lyase, 17-alpha-hydroxyprogesterone aldolase, CYPXVII, cytochrome P450 17A1, cytochrome P450, family 17, subfamily A, polypeptide 1, cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasia, cytochrome P450-C17, cytochrome P450c17, cytochrome p450 XVIIA1, steroid 17-alpha-monooxygenase
- HostRabbit
- IsotypeIgG
- Protein IDP05093
- Protein NameSteroid 17-alpha-hydroxylase/17,20 lyase
- ReactivityBovine, Equine, Human, Mouse, Rabbit, Rat, Sheep, Other Species
- Storage Instruction-20°C
- UNSPSC12352203
References
- Zhang W, Zhao Y, Zhang P, et al. Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable. Chemosphere. 2018,194:147-157. doi: 10.1016/j.chemosphere.2017.11.164Read this paper
- Han W, Xie W, Zhang Y, et al. Seasonal expression of P450c17 and 5α-reductase-2 in the scented gland of male muskrats (Ondatra zibethicus). Gen Comp Endocrinol. 2017,254:60-67. doi: 10.1016/j.ygcen.2017.09.015Read this paper
Datasheet
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