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CYP19 / Aromatase Antibody

LS-C747771
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetCYP19A1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CYP19 / Aromatase Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 24kDa/57kDa, while the observed MW by Western blot was 48kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:200), WB (1:1000 - 1:2000) The predicted MW is 24kDa/57kDa, while the observed MW by Western blot was 48kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1588
  • Target name
    CYP19A1
  • Target description
    cytochrome P450 family 19 subfamily A member 1
  • Target synonyms
    ARO, ARO1, CPV1, CYAR, CYP19, CYPXIX, P-450AROM, aromatase, cytochrome P-450AROM, cytochrome P450 19A1, cytochrome P450, family 19, subfamily A, polypeptide 1, cytochrome P450, subfamily XIX (aromatization of androgens), estrogen synthase, estrogen synthetase, flavoprotein-linked monooxygenase, microsomal monooxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Aromatase using anti-Aromatase antibody (A00071). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aromatase antigen affinity purified polyclonal antibody (Catalog # A00071) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aromatase at approximately 58KD. The expected band size for Aromatase is at 58KD.
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