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Western Blot analysis of 293 cells using CYP19A1 Polyclonal Antibody
Western Blot analysis of 293 cells using CYP19A1 Polyclonal Antibody
Western Blot analysis of 293 cells using CYP19A1 Polyclonal Antibody

CYP19A1 Antibody

CSB-PA001927
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCYP19A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CYP19A1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1588
  • Target name
    CYP19A1
  • Target description
    cytochrome P450 family 19 subfamily A member 1
  • Target synonyms
    ARO, ARO1, CPV1, CYAR, CYP19, CYPXIX, P-450AROM, aromatase, cytochrome P-450AROM, cytochrome P450 19A1, cytochrome P450, family 19, subfamily A, polypeptide 1, cytochrome P450, subfamily XIX (aromatization of androgens), estrogen synthase, estrogen synthetase, flavoprotein-linked monooxygenase, microsomal monooxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11511
  • Protein Name
    Aromatase
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of Aromatase using anti-Aromatase antibody (A00071). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aromatase antigen affinity purified polyclonal antibody (Catalog # A00071) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aromatase at approximately 58KD. The expected band size for Aromatase is at 58KD.
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