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Western Blot analysis of HT29 cells using CYP1A1/2 Polyclonal Antibody
Western Blot analysis of HT29 cells using CYP1A1/2 Polyclonal Antibody
Western Blot analysis of HT29 cells using CYP1A1/2 Polyclonal Antibody

CYP1A1/CYP1A2 Antibody

CSB-PA001929
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCYP1A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CYP1A1/CYP1A2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1543
  • Target name
    CYP1A1
  • Target description
    cytochrome P450 family 1 subfamily A member 1
  • Target synonyms
    AHH; AHRR; aryl hydrocarbon hydroxylase; CP11; CYP1; CYPIA1; cytochrome P1-450, dioxin-inducible; cytochrome P450 1A1; cytochrome P450 form 6; cytochrome P450, family 1, subfamily A, polypeptide 1; cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1; cytochrome P450-C; cytochrome P450-P1; flavoprotein-linked monooxygenase; hydroperoxy icosatetraenoate dehydratase; P1-450; P450-C; P450DX; xenobiotic monooxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04798
  • Protein Name
    Cytochrome P450 1A1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CYP1A1 using anti-CYP1A1 antibody (PB9544). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Lung Tissue Lysate Lane 2: Mouse Lung Tissue Lysate Lane 3: Human Placenta Tissue Lysate Lane 4: JURKAT Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP1A1 antigen affinity purified polyclonal antibody (Catalog # PB9544) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP1A1 at approximately 58KD. The expected band size for CYP1A1 is at 58KD.
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