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CYP3A4 / Cytochrome P450 3A4 Antibody

LS-C748532
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetCYP3A4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CYP3A4 / Cytochrome P450 3A4 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 57kDa, while the observed MW by Western blot was 50kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 57kDa, while the observed MW by Western blot was 50kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1576
  • Target name
    CYP3A4
  • Target description
    cytochrome P450 family 3 subfamily A member 4
  • Target synonyms
    CP33, CP34, CYP3A, CYP3A3, CYPIIIA3, CYPIIIA4, HLP, NF-25, P450C3, P450PCN1, VDDR3, cytochrome P450 3A4, 1,4-cineole 2-exo-monooxygenase, 1,8-cineole 2-exo-monooxygenase, P450-III, steroid inducible, albendazole monooxygenase (sulfoxide-forming), albendazole sulfoxidase, cholesterol 25-hydroxylase, cytochrome P450 3A3, cytochrome P450 HLp, cytochrome P450 NF-25, cytochrome P450, family 3, subfamily A, polypeptide 4, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4, cytochrome P450-PCN1, glucocorticoid-inducible P450, nifedipine oxidase, quinine 3-monooxygenase, taurochenodeoxycholate 6-alpha-hydroxylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cytochrome P450 3A4/CYP3A4 using anti-Cytochrome P450 3A4/CYP3A4 antibody (A00339-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytochrome P450 3A4/CYP3A4 antigen affinity purified polyclonal antibody (Catalog # A00339-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytochrome P450 3A4/CYP3A4 at approximately 57 kDa. The expected band size for Cytochrome P450 3A4/CYP3A4 is at 57 kDa.
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