Bio-Connect

Cystatin A antibody

GTX130885
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetCSTA
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    Cystatin A antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.78 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1475
  • Target name
    CSTA
  • Target description
    cystatin A
  • Target synonyms
    AREI; cystatin A (stefin A); cystatin AS; cystatin-A; PSS4; STF1; STFA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01040
  • Protein Name
    Cystatin-A
  • Scientific Description
    The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetCSTA
  • SizePrice
Anti-Cystatin A Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetCSTA
  • SizePrice
Product group Antibodies
Assay Genie
TargetCSTA
  • SizePrice
Western Blot analysis of HT29 cells using Cystatin A Polyclonal Antibody
Product group Antibodies
Cusabio
CSTA AntibodyCSB-PA002008
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCSTA
  • SizePrice
Product group Antibodies
Bioss Antibodies
ApplicationsFlow Cytometry, Western Blot
TargetCSTA
  • SizePrice
Figure 1. Western blot analysis of Cystatin A/CSTA using anti-Cystatin A/CSTA antibody (PB9564). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin A/CSTA antigen affinity purified polyclonal antibody (Catalog # PB9564) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystatin A/CSTA at approximately 14KD. The expected band size for Cystatin A/CSTA is at 11KD.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetCSTA
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetCSTA
  • SizePrice
ICC/IF analysis of HeLa cells using GTX57681 Cystatin A antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetCSTA
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot
TargetCSTA
  • SizePrice