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Western Blot analysis of HT29 cells using Cystatin A Polyclonal Antibody
Western Blot analysis of HT29 cells using Cystatin A Polyclonal Antibody
Western Blot analysis of HT29 cells using Cystatin A Polyclonal Antibody

CSTA Antibody

CSB-PA002008
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCSTA
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Overview

  • Supplier
    Cusabio
  • Product Name
    CSTA Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1475
  • Target name
    CSTA
  • Target description
    cystatin A
  • Target synonyms
    AREI, PSS4, STF1, STFA, cystatin-A, cystatin A (stefin A), cystatin AS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01040
  • Protein Name
    Cystatin-A
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cystatin A/CSTA using anti-Cystatin A/CSTA antibody (PB9564). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin A/CSTA antigen affinity purified polyclonal antibody (Catalog # PB9564) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystatin A/CSTA at approximately 14KD. The expected band size for Cystatin A/CSTA is at 11KD.
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