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ProductsBack/Cytochrome C Polyclonal Antibody
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) 1:300 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) 1:300 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) 1:300 followed by conjugation to the secondary antibody and DAB staining

Cytochrome C Polyclonal Antibody

BS-0013R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Equine, Mouse, Porcine, Rat
TargetCYCS
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Overview

  • Supplier
    Bioss
  • Product Name
    Cytochrome C Polyclonal Antibody
  • Delivery Days Customer
    7
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID54205
  • Target name
    CYCS
  • Target description
    cytochrome c, somatic
  • Target synonyms
    CYC, HCS, THC4, cytochrome c
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP99999
  • Protein Name
    Cytochrome c
  • Reactivity
    Bovine, Equine, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • Excessive Cu2+ deteriorates arsenite-induced apoptosis in chicken brain and resulting in immunosuppression, not in homeostasis. Wang Y et al., 2020 Jan, Chemosphere
    Read this paper
  • Ginsenoside Rg3 Prevents Cognitive Impairment by Improving Mitochondrial Dysfunction in the Rat Model of Alzheimers Disease. Zhang Y et al., 2019 Sep 11, J Agric Food Chem
    Read this paper
  • Cytoplasmic upregulation of Cyto c and AIF serve as biomarkers of mechanical asphyxia death. Zhang H et al., 2019, Am J Transl Res
    Read this paper
  • The hepatoprotective effect of polysaccharides from Pleurotus ostreatus on carbon tetrachloride-induced acute liver injury rats. Zhu B et al., 2019 Jun 15, Int J Biol Macromol
    Read this paper
  • Copper induces oxidative stress and apoptosis through mitochondria-mediated pathway in chicken hepatocytes. Yang F et al., 2019 Feb, Toxicol In Vitro
    Read this paper
  • Lupeol against high-glucose-induced apoptosis via enhancing the anti-oxidative stress in rabbit nucleus pulposus cells. Guo MB et al., 2018 Oct, Eur Spine J
    Read this paper
  • MicroRNA-193b-3p regulates hepatocyte apoptosis in selenium-deficient broilers by targeting MAML1. Liu T et al., 2018 Sep, J Inorg Biochem
    Read this paper
  • Copper-Mediated Mitochondrial Fission/Fusion Is Associated with Intrinsic Apoptosis and Autophagy in the Testis Tissues of Chicken. Shao Y et al., 2019 Apr, Biol Trace Elem Res
    Read this paper
  • Effects of HSYA on the proliferation and apoptosis of MSCs exposed to hypoxic and serum deprivation conditions. Song X et al., 2018 Jun, Exp Ther Med
    Read this paper
  • Phosphoprotein Gene Contributes to the Enhanced Apoptosis Induced by Wild-Type Rabies Virus GD-SH-01 In Vitro. Tian Q et al., 2017, Front Microbiol
    Read this paper

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Figure 1. Western blot analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (PB9334). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytochrome c/CYCS antigen affinity purified polyclonal antibody (PB9334) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytochrome c/CYCS at approximately 14 kDa. The expected band size for Cytochrome c/CYCS is at 12 kDa.
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