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ProductsBack/Cytochrome P450 Reductase (POR) Mouse Monoclonal Antibody [Clone ID: OTI5B3]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY POR (Cat# RC202172, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POR antibody (Cat# TA500612). Positive lysates [LY424436] (100ug) and [LC424436] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY POR (Cat# RC202172, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POR antibody (Cat# TA500612). Positive lysates [LY424436] (100ug) and [LC424436] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY POR (Cat# RC202172, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POR antibody (Cat# TA500612). Positive lysates [LY424436] (100ug) and [LC424436] (20ug) can be purchased separately from OriGene.

Cytochrome P450 Reductase (POR) Mouse Monoclonal Antibody [Clone ID: OTI5B3]

CF500612
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPOR
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Cytochrome P450 Reductase (POR) Mouse Monoclonal Antibody [Clone ID: OTI5B3]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5B3
  • Gene ID5447
  • Target name
    POR
  • Target description
    cytochrome p450 oxidoreductase
  • Target synonyms
    CPR, CYPOR, P450R, NADPH--cytochrome P450 reductase, NADPH--hemoprotein reductase, NADPH-dependent cytochrome P450 reductase, P450 (cytochrome) oxidoreductase
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP16435
  • Protein Name
    NADPH--cytochrome P450 reductase
  • Scientific Description
    Carrier-free (BSA/glycerol-free) POR mouse monoclonal antibody, clone OTI5B3 (formerly 5B3)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of POR using anti-POR antibody (PB9736). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POR antigen affinity purified polyclonal antibody (Catalog # PB9736) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POR at approximately 77 kDa. The expected band size for POR is at 77 kDa.
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