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Western blot All lanes: POR antibody at 2microg/ml Lane 1: K562 whole cell lysate Lane 2: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 77 kDa Observed band size: 77 kDa
Western blot All lanes: POR antibody at 2microg/ml Lane 1: K562 whole cell lysate Lane 2: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 77 kDa Observed band size: 77 kDa
Western blot All lanes: POR antibody at 2microg/ml Lane 1: K562 whole cell lysate Lane 2: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 77 kDa Observed band size: 77 kDa

POR Antibody

CSB-PA06275A0RB
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPOR
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Overview

  • Supplier
    Cusabio
  • Product Name
    POR Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5447
  • Target name
    POR
  • Target description
    cytochrome p450 oxidoreductase
  • Target synonyms
    CPR; CYPOR; NADPH--cytochrome P450 reductase; NADPH-dependent cytochrome P450 reductase; NADPH--hemoprotein reductase; P450 (cytochrome) oxidoreductase; P450R
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16435
  • Protein Name
    NADPH--cytochrome P450 reductase
  • Scientific Description
    This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of POR using anti-POR antibody (PB9736). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POR antigen affinity purified polyclonal antibody (Catalog # PB9736) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POR at approximately 77 kDa. The expected band size for POR is at 77 kDa.
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