Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Cytokeratin 10 antibody (GTX113221) diluted at 1:10000 and competitor's antibody (sc-15367) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Cytokeratin 10 antibody (GTX113221) diluted at 1:10000 and competitor's antibody (sc-15367) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Cytokeratin 10 antibody
GTX113221
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetKRT10
Overview
- SupplierGeneTex
- Product NameCytokeratin 10 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:5000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3858
- Target nameKRT10
- Target descriptionkeratin 10
- Target synonymsBCIE, BIE, CK10, EHK, EHK2, EHK2A, EHK2B, IHL, K10, KPP, keratin, type I cytoskeletal 10, CK-10, cytokeratin 10, keratin 10, type I
- HostRabbit
- IsotypeIgG
- Protein IDP13645
- Protein NameKeratin, type I cytoskeletal 10
- Scientific DescriptionThis gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Wang S, Qiu L, Meng X, et al. Knock-down of filaggrin influences the mitogen-activated protein kinases signaling pathway in normal human epidermal keratinocytes. Med Sci (Paris). 2018,34 Focus issue F1:94-98. doi: 10.1051/medsci/201834f116Read this paper
Datasheet
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