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WB analysis of various sample lysates using GTX66286 Cytokeratin 12 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66286 Cytokeratin 12 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66286 Cytokeratin 12 antibody. Dilution : 1:1000 Loading : 25microg per lane

Cytokeratin 12 antibody

GTX66286
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetKRT12
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Overview

  • Supplier
    GeneTex
  • Product Name
    Cytokeratin 12 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3859
  • Target name
    KRT12
  • Target description
    keratin 12
  • Target synonyms
    K12, MECD1, keratin, type I cytoskeletal 12, CK-12, cytokeratin-12, keratin 12, type I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99456
  • Protein Name
    Keratin, type I cytoskeletal 12
  • Scientific Description
    KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KRT12 using anti-KRT12 antibody (M05227). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT12 antigen affinity purified monoclonal antibody (Catalog # M05227) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRT12 at approximately 54 kDa. The expected band size for KRT12 is at 54 kDa.
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