
Immunohistochemical analysis of paraffin-embedded HT29 xenograft , using Cytokeratin 20(GTX100693) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Cytokeratin 20 antibody [C2C3], C-term
GTX100693
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetKRT20
Overview
- SupplierGeneTex
- Product NameCytokeratin 20 antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID54474
- Target nameKRT20
- Target descriptionkeratin 20
- Target synonymsCD20, CK-20, CK20, K20, KRT21, keratin, type I cytoskeletal 20, cytokeratin 20, keratin 20, type I, protein IT
- HostRabbit
- IsotypeIgG
- Protein IDP35900
- Protein NameKeratin, type I cytoskeletal 20
- Scientific DescriptionThe protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 20 antibody [C2C3], C-term (GTX100693) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 20 antibody [C2C3], C-term (GTX100693) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100693/GTX100693_39995_20231103_WB_TPM_watermark_23110819_786.webp)

![IHC-P analysis of human colon carcinoma section using GTX02660 Cytokeratin 20 antibody [KRT20/3129R].](https://www.genetex.com/upload/website/prouct_img/normal/GTX02660/GTX02660_20210319_IHC-P_w_23053122_842.webp)
![IHC-P analysis of human mucosa from descending colon tissue using GTX04376 Cytokeratin 20 antibody [MSVA-620R] HistoMAX?. In the colon mucosa cytokeratin 20 staining is strongest at the surface epithelium and decreased towards the crypt base.](https://www.genetex.com/upload/website/prouct_img/normal/GTX04376/GTX04376_20230728_IHC-P_41_23072722_211.webp)



![IHC-P analysis of human colon carcinoma tissue using GTX73572 Cytokeratin 20 antibody [KS 20.8] (ready-to-use).](https://www.genetex.com/upload/website/prouct_img/normal/GTX73572/GTX73572_20191203_IHC-P_169_w_23061322_885.webp)
![IHC-P analysis of human colon carcinoma tissue using GTX73825 Cytokeratin 20 antibody [KS 20.8].](https://www.genetex.com/upload/website/prouct_img/normal/GTX73825/GTX73825_20191203_IHC-P_200_w_23061322_642.webp)