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ProductsBack/Cytokeratin 5 antibody [GT1486]
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cytokeratin 5 antibody [GT1486] (GTX634308) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cytokeratin 5 antibody [GT1486] (GTX634308) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cytokeratin 5 antibody [GT1486] (GTX634308) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Cytokeratin 5 antibody [GT1486]

GTX634308
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKRT5
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Overview

  • Supplier
    GeneTex
  • Product Name
    Cytokeratin 5 antibody [GT1486]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT1486
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3852
  • Target name
    KRT5
  • Target description
    keratin 5
  • Target synonyms
    CK5, DDD, DDD1, EBS1, EBS2, EBS2A, EBS2B, EBS2C, EBS2D, EBS2E, EBS2F, K5, KRT5A, keratin, type II cytoskeletal 5, 58 kda cytokeratin, CK-5, cytokeratin-5, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types), keratin 5, type II, type-II keratin Kb5
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP13647
  • Protein Name
    Keratin, type II cytoskeletal 5
  • Scientific Description
    The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cytokeratin 5 using anti-Cytokeratin 5 antibody (A00398). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 5 antigen affinity purified polyclonal antibody (Catalog # A00398) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 5 at approximately 62 kDa. The expected band size for Cytokeratin 5 is at 62 kDa.
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