ICC/IF analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using GTX83140 Cytokeratin 8 antibody [8A5D12]. Green : Cytokeratin 8 Blue: DRAQ5 fluorescent DNA dye
![IHC-P analysis of human Kidney tissue using GTX83140 Cytokeratin 8 antibody [8A5D12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83140/GTX83140_20170912_IHC-P_1_w_23061322_138.webp "IHC-P analysis of human Kidney tissue using GTX83140 Cytokeratin 8 antibody [8A5D12].")
![IHC-P analysis of human breast carcinoma (A), lung cancer (B) and ovarian cancer tissue (C) using GTX83140 Cytokeratin 8 antibody [8A5D12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83140/GTX83140_20170912_IHC-P_w_23061322_386.webp "IHC-P analysis of human breast carcinoma (A), lung cancer (B) and ovarian cancer tissue (C) using GTX83140 Cytokeratin 8 antibody [8A5D12].")
![WB analysis of A549 (1), HeLa (2), MCF-7 (3), A431 (4) and HepG2 (5) cell lysate using GTX83140 Cytokeratin 8 antibody [8A5D12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83140/GTX83140_20170912_WB_w_23061322_371.webp "WB analysis of A549 (1), HeLa (2), MCF-7 (3), A431 (4) and HepG2 (5) cell lysate using GTX83140 Cytokeratin 8 antibody [8A5D12].")
ICC/IF analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using GTX83140 Cytokeratin 8 antibody [8A5D12]. Green : Cytokeratin 8 Blue: DRAQ5 fluorescent DNA dye
Cytokeratin 8 antibody [8A5D12]
GTX83140
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetKRT8
Overview
- SupplierGeneTex
- Product NameCytokeratin 8 antibody [8A5D12]
- Delivery Days Customer9
- Application Supplier NoteWB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID8A5D12
- ConjugateUnconjugated
- Gene ID3856
- Target nameKRT8
- Target descriptionkeratin 8
- Target synonymsCARD2; CK8; CK-8; CYK8; cytokeratin-8; K2C8; K8; keratin 8, type II; keratin, type II cytoskeletal 8; KO; type-II keratin Kb8
- HostMouse
- IsotypeIgG1
- Protein IDP05787
- Protein NameKeratin, type II cytoskeletal 8
- Scientific DescriptionThis gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203