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Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) Polyclonal Antibody

CAU26313
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetCtla4
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Overview

  • Supplier
    Biomatik
  • Product Name
    Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.85 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID12477
  • Target name
    Ctla4
  • Target description
    cytotoxic T-lymphocyte-associated protein 4
  • Target synonyms
    Cd152; CD152 antigen; Ctla; Ctla-4; cytotoxic T-lymphocyte protein 4; cytotoxic T-lymphocyte-associated antigen 4; Ly-5; Ly-56
  • Host
    Rabbit
  • Protein IDP09793
  • Protein Name
    Cytotoxic T-lymphocyte protein 4
  • Scientific Description
    The Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CTLA4 using anti-CTLA4 antibody (A00020-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH3T3 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTLA4 antigen affinity purified polyclonal antibody (Catalog # A00020-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTLA4 at approximately 25KD. The expected band size for CTLA4 is at 25KD.
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