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CTLA4 antibody

GTX134527
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCtla4
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Overview

  • Supplier
    GeneTex
  • Product Name
    CTLA4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.83 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID12477
  • Target name
    Ctla4
  • Target description
    cytotoxic T-lymphocyte-associated protein 4
  • Target synonyms
    Cd152; CD152 antigen; Ctla; Ctla-4; cytotoxic T-lymphocyte protein 4; cytotoxic T-lymphocyte-associated antigen 4; Ly-5; Ly-56
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09793
  • Protein Name
    Cytotoxic T-lymphocyte protein 4
  • Scientific Description
    This gene is a member of the immunoglobulin superfamily, and encodes a protein that functions as a negative regulator of T-cell responses. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CTLA4 using anti-CTLA4 antibody (A00020-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH3T3 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTLA4 antigen affinity purified polyclonal antibody (Catalog # A00020-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTLA4 at approximately 25KD. The expected band size for CTLA4 is at 25KD.
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WB analysis of purified CTLA4 recombinant protein using GTX14005 CTLA4 antibody [9H10]. Lane 1 : 0.2 ug reduced purified mouse CTLA-4 with histidine tag at C-terminus Lane 2 : 0.1 ug reduced purified mouse CTLA-4 with histidine tag at C-terminus Lane 3 : 0.05 ug reduced purified mouse CTLA-4 with histidine tag at C-terminus Dilution : 8 ug/ml
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WB analysis of purified CTLA4 recombinant protein using GTX14132 CTLA4 antibody [9D9]. Lane 1 : 1 ug reduced purified mouse CTLA-4 with histidine tag at C-terminus Lane 2 : 0.5 ug reduced purified mouse CTLA-4 with histidine tag at C-terminus Dilution : 8 ug/ml
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