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DARS2 Polyclonal Antibody

RD84731A
Reddot Biotech
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetDARS2
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    DARS2 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID55157
  • Target name
    DARS2
  • Target description
    aspartyl-tRNA synthetase 2, mitochondrial
  • Target synonyms
    ASPRS, LBSL, MT-ASPRS, mtAspRS, aspartate--tRNA ligase, mitochondrial, aspartate tRNA ligase 2, mitochondrial, aspartyl-tRNA synthetase, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL).
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DARS2 using anti-DARS2 antibody (A06034-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DARS2 antigen affinity purified polyclonal antibody (Catalog # A06034-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DARS2 at approximately 74KD. The expected band size for DARS2 is at 74KD.
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