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ProductsBack/Daxx Antibody (YA2854, Rabbit)

Daxx Antibody (YA2854, Rabbit)

HY-P83109
MedChem Express
TargetDAXX
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Daxx Antibody (YA2854, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1616
  • Target name
    DAXX
  • Target description
    death domain associated protein
  • Target synonyms
    BING2, DAP6, EAP1, SMIM40, death domain-associated protein 6, CENP-C binding protein, ETS1-associated protein 1, Fas-binding protein, death-associated protein 6, fas death domain-associated protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Daxx Antibody (YA2854) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2854), targeting Daxx, with a predicted molecular weight of 81 kDa (observed band size: 110 kDa). Daxx Antibody (YA2854) can be used for WB, ICC/IF experiment in human background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Daxx using anti-Daxx antibody (PB9550). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Daxx antigen affinity purified polyclonal antibody (Catalog # PB9550) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Daxx at approximately 115 kDa. The expected band size for Daxx is at 81 kDa.
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